Functional Expression of Carnitine/Organic Cation Transporter 1 in Murine Choroid Plexus.
Biol Pharm Bull
; 47(9): 1484-1486, 2024.
Article
em En
| MEDLINE
| ID: mdl-39231688
ABSTRACT
Membrane transporters expressed in the choroid plexus (CP) are involved in the transport of substances between the blood and cerebrospinal fluid (CSF). Carnitine/organic cation transporter 1 (OCTN1, also known as SLC22A4) is expressed in rodent CP; however, its specific roles in blood-CSF transport remain unclear. Therefore, in this study, we aimed to evaluate the potential role of OCTN1 in the elimination of substances from CSF. Tritium-labeled ergothioneine ([3H]ERGO), a typical in vivo substrate of OCTN1, was injected into the lateral ventricles of wild-type and octn1 gene knockout (octn1-/-) mice. Clearance of [3H]ERGO from CSF was higher than that of the bulk flow marker, [14C]mannitol, in wild-type mice. However, [3H]ERGO clearance was significantly lower in octn1-/- mice than in wild-type mice. Furthermore, OCTN1 expression in CP was determined via immunohistochemical analysis. CP/CSF ratio of [3H]ERGO was significantly lower in octn1-/- mice than in wild-type mice. These results suggest that OCTN1 is functionally expressed in CP and involved in the elimination of ERGO from CSF in mice.
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Base de dados:
MEDLINE
Assunto principal:
Plexo Corióideo
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Camundongos Knockout
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Proteínas de Transporte de Cátions Orgânicos
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Ergotioneína
Limite:
Animals
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article