Mass Spectrometry Imaging for the Characterization of CâC Localization in Unsaturated Lipid Isomers at the Single-Cell Level.
Anal Chem
; 2024 Sep 13.
Article
em En
| MEDLINE
| ID: mdl-39269953
ABSTRACT
Unsaturated lipids with carbon-carbon double bonds (CâC) have been implicated in the pathogenesis of various diseases. While mass spectrometry imaging (MSI) has been employed to map the distribution of lipid isomers in tissue sections, the identification of lipid CâC positional isomers at the single-cell level using MSI poses a significant challenge. In this study, we developed a novel approach utilizing ToF-SIMS in conjunction with the Paternò-Büchi (P-B) photochemical reaction to characterize the CâC localization in unsaturated lipid isomers at the single-cell level. The P-B reaction was employed to produce adduct products, which were subsequently subjected to collision-induced dissociation by the primary ion beam of ToF-SIMS to generate characteristic ion pairs indicative of the presence of CâC bonds. Utilizing this approach, lipid isomers in brain and skeletal tissues from mice, as well as different cell lines, were visualized at single-cell resolution. Furthermore, distinct variations in the composition of FA 181 isomers across different microregions and cell types were revealed. Our P-B ToF-SIMS approach enables the accurate identification and characterization of complex lipid structures with remarkable spatial resolution and can be helpful in understanding the physiological role of these CâC positional isomers.
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MEDLINE
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En
Ano de publicação:
2024
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Article