Development and evaluation of a loop-mediated isothermal amplification (LAMP) method for Candida glabrata detection.

ABSTRACT

PURPOSE: Loop-mediated isothermal amplification (LAMP) is a simple and rapid nucleic acid method for DNA amplification at a constant temperature. The "gold standard" culture method for yeast detection, has low sensitivity with severe consequences, increasing morbidity and mortality rates. Here, we report the development of a LAMP method for the specific detection of C. glabrata. METHODOLOGY: The specific LAMP primers for C. glabrata detection were designed and evaluated. RESULTS: The LAMP assay accurately detected C. glabrata with no cross-reactivity with other Candida species. CONCLUSION: The developed molecular method would be a promising tool in the management of invasive candidiasis.