Vasopressin-stimulated phosphorylation of rat liver phospholipid methyltransferase in isolated hepatocytes.
FEBS Lett
; 196(2): 274-8, 1986 Feb 17.
Article
em En
| MEDLINE
| ID: mdl-3949001
ABSTRACT
Addition of vasopressin (1 microM) to isolated rat hepatocytes prelabeled with [32P]phosphate was accompanied by a 250% increase in the phosphorylation of phospholipid methyltransferase. Vasopressin-stimulated phospholipid methyltransferase phosphorylation was time- and dose-dependent. 32P-labeled phospholipid methyltransferase was recovered by immunoprecipitation and SDS-polyacrylamide gel electrophoresis. After electrophoresis, phospholipid methyltransferase was electroeluted from the polyacrylamide gel and subjected to tryptic digestion or HCl hydrolysis. Analysis of 32P-labeled peptides reveals only one site of phosphorylation and the analysis of [32P]phosphoamino acids indicates that phosphoserine is the only labeled amino acid.
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Base de dados:
MEDLINE
Assunto principal:
Vasopressinas
/
Fígado
/
Metiltransferases
Limite:
Animals
Idioma:
En
Ano de publicação:
1986
Tipo de documento:
Article