A 13C NMR method for determination of the transbilayer distribution of phosphatidylcholine in large, unilamellar, protein-free and protein-containing vesicles.
Biochim Biophys Acta
; 551(2): 248-59, 1979 Mar 08.
Article
em En
| MEDLINE
| ID: mdl-420832
ABSTRACT
(1) Large unilamellar vesicles have been prepared from N-[Ne3-13C]-18 1c/18 1c-phosphatidylcholine, both with and without the major intrinsic proteins from the human erythrocyte membrane incorporated in the bilayer. (2) It is shown that the inside-outside distribution of the lipid molecules in these large unilamellar structures can be determined using 13C NMR. (3) Large vesicles of 18 1c/18 1c-phosphatidylcholine containing glycophorin show an enhanced permeability to Dy3+. It is shown that the permeability barrier of these vesicles can be restored by addition of 10 mol% 18 1c/18 1c-phosphatidylethanolamine or 1-18 1c-lysophosphatidylcholine.
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Base de dados:
MEDLINE
Assunto principal:
Fosfatidilcolinas
/
Membrana Eritrocítica
/
Eritrócitos
/
Proteínas de Membrana
/
Membranas Artificiais
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
1979
Tipo de documento:
Article