Freeze etching of cells without cryoprotectants.
J Cell Biol
; 53(1): 116-26, 1972 Apr.
Article
em En
| MEDLINE
| ID: mdl-4622424
ABSTRACT
The technique of spray-freeze etching was applied to unicellular organisms. The superior freezing rates obtainable with this method gave excellent cryofixation on Chlorella, Euglena, and spermatozoa without the use of antifreeze agents, and cell damage due to ice crystal formation was never observed. In many instances the resultant morphology differed significantly from that obtained from glycerol-treated, freeze-etched cells. Furthermore, viability studies of spray-frozen Chlorella compared favorably with cells frozen by other methods.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Espermatozoides
/
Técnicas Histológicas
/
Clorófitas
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Euglena gracilis
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
1972
Tipo de documento:
Article