Mutagenicity of actinomycin D in Neurospora crassa.
Mutat Res
; 33(2-3): 187-92, 1975 Dec.
Article
em En
| MEDLINE
| ID: mdl-55964
ABSTRACT
Actinomycin D is known to bind to native DNA and is widely used as an antineoplastic agent and inhibitor of DNA-dependent RNA and protein synthesis. We report here the induction by actinomycin D of purple adenine-requiring mutants (ad-3) in wild-type Neurospora crassa. A significant increase in the frequency of ad-3 mutants was evident when the organism was grown vegetatively in the presence of actinomycin D; the mutation frequency was at least 3.6 per 106 survivors. The actinomycin D-induced ad-3 mutants were 29% ad-3A and 71% ad-3B. The ad-3B mutants were classed by complementation pattern as 25% nonpolarized complementing; 14% polarized complementing; and 61% noncomplementing. The spectrum of complementation types of the actinomycin D-induced mutants most closely parallels that of mutants induced by ICR-170, known to induce base-pair insertions or deletions, or that of X ray-induced or spontaneous mutants. It is significantly different from spectra seen following treatment with nitrous acid or N-methyl-N'-nitro-N-nitrosoguanidine, agents known to induce mainly base-pair substitutions.
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Base de dados:
MEDLINE
Assunto principal:
Dactinomicina
/
Mutação
/
Neurospora
/
Neurospora crassa
Idioma:
En
Ano de publicação:
1975
Tipo de documento:
Article