Induction of a relatively fast transbilayer movement of phosphatidylcholine in vesicles. A 13CNMR study.
Biochim Biophys Acta
; 468(2): 318-26, 1977 Jul 14.
Article
em En
| MEDLINE
| ID: mdl-560207
ABSTRACT
[N-13CH3]Phosphatidylcholines are introduced into the outer monolayer of phosphatidylcholine vesicles with the phosphatidylcholine exchange protein from bovine liver. The transbilayer distribution of the [N-13CH3]phosphatidyl-choline is measured with 13C NMR. The transbilayer movements of [N-13CH3]-dioleoyl phosphatidylcholine and [N-13CH3]dimyristoyl phosphatidylcholine at 30 degrees C in vesicles composed of these phosphatidylcholines are extremely slow processes with estimated half-times of days. [N-13CH3]Dioleoyl phosphatidyl-choline introduced into dimyristoyl phosphatidylcholine vesicles migrates from the outer to the inner monolayer with a half-time of less than 12 h. The data suggest that differential changes in the lateral packing of the two monolayers might be a driving force for transbilayer transport of phospholipids.
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Base de dados:
MEDLINE
Assunto principal:
Fosfatidilcolinas
/
Espectroscopia de Ressonância Magnética
/
Proteínas de Transporte
/
Membranas Artificiais
Limite:
Animals
Idioma:
En
Ano de publicação:
1977
Tipo de documento:
Article