Partial purification of Simian virus 40 large T antigen by immunoaffinity chromatography and its detection by enzyme-linked immunosorbent assay.
Acta Med Okayama
; 38(4): 341-7, 1984 Aug.
Article
em En
| MEDLINE
| ID: mdl-6093443
ABSTRACT
Simian virus 40 (SV40) large T antigen was partially purified from small amounts of SV40-infected and SV40-transformed cells by immunoaffinity chromatography with high recovery. T antigen, in both crude and partially purified states, was detected rapidly by a sensitive and quantitative enzyme-linked immunosorbent assay (ELISA). Stability of the partially purified T antigen was found to increase by addition of 0.01% bovine serum albumin (BSA).
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Base de dados:
MEDLINE
Assunto principal:
Proteínas Virais
/
Vírus 40 dos Símios
/
Antígenos Virais de Tumores
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
1984
Tipo de documento:
Article