Colorimetric assay of urinary 5-hydroxy-3-indoleacetic acid.
Clin Biochem
; 15(2): 106-8, 1982 Apr.
Article
em En
| MEDLINE
| ID: mdl-6178531
ABSTRACT
We describe a method for 5-hydroxy-3-indoleacetic acid in urine based on its reaction with nitrosonaphthol in the presence of mercaptoethanolamine, an odorless compound. Mercaptoethanolamine shifts the color maximum from 540 nm to 640 nm, intensifies the color and discharges the color of interfering substances. The method is rapid and free from interferences.
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Base de dados:
MEDLINE
Assunto principal:
Ácido Hidroxi-Indolacético
Limite:
Adult
/
Humans
Idioma:
En
Ano de publicação:
1982
Tipo de documento:
Article