Preparation of a new fluorogenic substrate of alpha-amylases and a simple alpha-amylase assay by HPLC.
J Biochem
; 93(4): 1055-60, 1983 Apr.
Article
em En
| MEDLINE
| ID: mdl-6190796
ABSTRACT
A new substrate of alpha-amylases, O-6-deoxy-6-[(2-pyridyl)amino]-alpha-D-glucopyranosyl-(1 leads to 4)-O-alpha-D-glucopyranosyl-(1 leads to 4)-O-alpha-D-glucopyranosyl-(1 leads to 4)-O-alpha-D-glucopyranosyl-(1 leads to 4)-D-glucopyranose, was prepared using dextrin as a starting material. Compared with other substrates so far reported, the fluorogenic substrate is unique in that it is resistant to exo-alpha-glucosidases due to the blocking group introduced into the non-reducing end glucose residue. The product of alpha-amylase digestion was rapidly separated from the substrate and was detected very sensitively by HPLC and a fluorescence detector. This method for alpha-amylase assay was also applied for determination of alpha-amylase in human serum.
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Base de dados:
MEDLINE
Assunto principal:
Oligossacarídeos
/
Alfa-Amilases
/
Corantes Fluorescentes
/
Amilases
Idioma:
En
Ano de publicação:
1983
Tipo de documento:
Article