Localization of a 16,000-dalton fragment of the common precursor of adrenocorticotropin and beta-lipotropin in the rat and human pituitary gland.
J Cell Biol
; 86(3): 825-30, 1980 Sep.
Article
em En
| MEDLINE
| ID: mdl-6251097
ABSTRACT
To clearly identify cells and organelles containing the common precursor (31,000 dalton) for both adrenocorticotropin (ACTH) and beta-lipotropin (beta-LPH), an immunohistochemical localization of a fragment (16,000 dalton) of the precursor that is not common to beta-LPH and ACTH was conducted in rat and human pituitary glands. With the help of specific antibodies that do not cross-react with beta-LPH and ACTH, the 16,000-dalton fragment was localized in the cells that also produce ACTH and beta-LPH in both the pars distalis and pars intermedia of the rat pituitary. At the electron microscope level, the secretory granules that contain ACTH were also stained for 16,000-dalton fragment. In the human pituitary, the 16,000-dalton fragment was also observed in all the secretory granules of lipocorticotrophs. These results suggest that, after enzymatic cleavage, fragment(s) of the common precursor and/or the whole common precursor remain packaged within the secretory granules with peptides of known activity.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Hipófise
/
Precursores de Proteínas
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Beta-Lipotropina
/
Hormônio Adrenocorticotrópico
Limite:
Animals
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Humans
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Male
Idioma:
En
Ano de publicação:
1980
Tipo de documento:
Article