The effect of amiloride on hormonal regulation of amino acid transport in isolated and cultured adult rat hepatocytes.
Biochim Biophys Acta
; 642(1): 88-95, 1981 Mar 20.
Article
em En
| MEDLINE
| ID: mdl-6261811
ABSTRACT
Insulin and glucagon stimulate amino acid transport in isolated rat hepatocytes. Amiloride, a specific Na+-influx inhibitor, completely inhibited the hormonal (glucagon or insulin) stimulation of alpha-aminoisobutyric acid influx by preventing the emergence of a high-affinity transport component. The drug also inhibited [14C]valine incorporation into hepatocyte protein. The half-maximal concentration of amiloride for inhibition of protein synthesis was similar to that required for inhibition of hormone-stimulated amino acid transport (approx. 0.1 mM). In primary cultured rat hepatocytes, amiloride markedly depressed the stimulation of alpha-aminoisobutyric acid transport by glucagon, or a mixture of glucagon, insulin and epidermal growth factor. These results suggest that amiloride inhibits the hormonal stimulation of hepatocyte amino acid transport by preventing the synthesis of high-affinity transport proteins. They also suggest that the hormonal stimulation of hepatocyte amino acid transport is dependent, at least partly, on Na+ influx.
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Base de dados:
MEDLINE
Assunto principal:
Pirazinas
/
Glucagon
/
Amilorida
/
Ácidos Aminoisobutíricos
/
Insulina
/
Fígado
Limite:
Animals
Idioma:
En
Ano de publicação:
1981
Tipo de documento:
Article