Possible role of non-bilayer lipids in the structure of mitochondria. A freeze-fracture electron microscopy study.

ABSTRACT

The possible role of non-bilayer phospholipids on the structure of isolated rat liver mitochondria has been morphologically studied. Freshly isolated freeze-fractured mitochondria show smooth fracture faces with particles, representing the limiting membranes. The frequency and size of the particles is representative for the various membrane faces. Distinctly large particles and pits represent the attachment sites of cristae to the inner membrane. Liposome-like structures in the matrix are found upon incubation with Ca2+ and Mn2+. At 5 mM Mn2+ and more, curved hexagonal (HII) phase tubes are observed. Subsequent addition of 1 mM EDTA results in disappearance of the HII tubes, and liposomal structures can again be seen. These findings are interpreted in terms of an Mn2+-induced lamellar to HII phase transition. Patchwork-like structures characterize the membranes of mitochondria, quenched from 37 degrees C, as well as those incubated with Ca2+, Mn2+, Mg2+ and apo- or cytochrome c. This phenomenon is interpreted as being the result of the fracture plane, jumping from the outer to the inner limiting membrane and vice versa at sites of contact. A semi-fusion model, in which non-bilayer lipids are involved, is proposed for these contact sites.