Extraction of SV40 T-antigen from lysates of transformed cells.
FEBS Lett
; 168(1): 129-33, 1984 Mar 12.
Article
em En
| MEDLINE
| ID: mdl-6323217
ABSTRACT
Subcellular fractions from SV-40 transformed hamster lens cells, prepared by chemical extractions, were tested for the presence of T-antigen by immunoautoradiography. Most of the T-antigen was present in the nucleus and was resistant to extraction by 2 M NaCl, indicating an association with the nuclear matrix. Another part of the T-antigen was, under certain conditions, resistant to extraction of the cells with a nonionic detergent. This T-antigen could be solubilized by Ca2+ at low temperature, conditions that also cause a specific depolymerization of microtubules.
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Base de dados:
MEDLINE
Assunto principal:
Transformação Celular Viral
/
Vírus 40 dos Símios
/
Antígenos Virais de Tumores
Limite:
Animals
Idioma:
En
Ano de publicação:
1984
Tipo de documento:
Article