Morphology of bovine viral diarrhea virus.
Am J Vet Res
; 45(5): 845-50, 1984 May.
Article
em En
| MEDLINE
| ID: mdl-6329046
ABSTRACT
The morphology of bovine viral diarrhea virus (BVDV) was studied by electron microscopy. The NADL strain of BVDV was plaque purified 3 times, concentrated by polyethylene glycol precipitation, and purified by centrifugation to equilibrium in continuous potassium tartrate density-gradients. The virus was examined by negative-stain electron microscopy in the presence or absence of specific antiserum. The density of BVDV was between 1.101 g/cm3 and 1.174 g/cm3, with the peak at maximum infectivity at 1.122 g/cm3. Oval to pleomorphic viral particles, 120 ( +/- 30) nm in diameter, were enriched in the peak of maximum infectivity. The detailed structure of virions was revealed a 5- to 7-microns thick unit membrane-like envelope layer with numerous projecting knobs, 4 to 5 nm in diameter, surrounding an interior core-like structure. Viral particles measuring 120 ( +/- 30) nm were found in large aggregates in the presence of specific antiserum.
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Base de dados:
MEDLINE
Assunto principal:
Vírion
/
Pestivirus
/
Vírus da Diarreia Viral Bovina
Limite:
Animals
Idioma:
En
Ano de publicação:
1984
Tipo de documento:
Article