The use of cytocentrifuge preparations for the demonstration of T cell surface antigens.
J Immunol Methods
; 66(2): 327-30, 1984 Feb 10.
Article
em En
| MEDLINE
| ID: mdl-6361154
ABSTRACT
Cytocentrifuged cell preparations have been evaluated as substrates for the demonstration by immunofluorescence of T cell surface antigens. Results obtained by a 2-stage method with fluorescent conjugate specific for the isotype of the first stage monoclonal antibody, and by a 3-stage procedure employing an intermediate antiserum to mouse IgG as amplifying agent, showed good correlation with those obtained by a standard technique performed on cells in suspension. These methods have the advantages of requiring fewer cells and less antibody and the cytocentrifuge preparations may be stored for batch assay.
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Base de dados:
MEDLINE
Assunto principal:
Linfócitos T
/
Imunofluorescência
/
Antígenos de Superfície
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
1984
Tipo de documento:
Article