Presence of genomic polyadenylate and absence of detectable virion transcriptase in human coronavirus OC-43.
J Gen Virol
; 39(1): 29-39, 1978 Apr.
Article
em En
| MEDLINE
| ID: mdl-641531
ABSTRACT
Human coronavirus RNA, prepared by extraction of purified virions with phenol-chloroform, consists of a major 15 to 55S class and a minor 4S class of RNA fragments. Polyadenylic acid [poly (A)] sequences are present in 15 to 55S but not in 4S RNA, suggesting different functions for each class. A stretch of poly (A) of approximately 19 adenosine monophosphate residues was obtained in sizing experiments after digesting OC-43 RNA with pancreatic and T1 ribonucleases. An OC-43 virion RNA transcriptase could not be detected with systems optimal for detecting the transcriptases of influenza and Newcastle disease virus.
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Base de dados:
MEDLINE
Assunto principal:
Poli A
/
RNA Polimerases Dirigidas por DNA
/
RNA Viral
/
Coronaviridae
Idioma:
En
Ano de publicação:
1978
Tipo de documento:
Article