Molecular mass analysis of murine immunosuppressive immunoglobulin G-binding factors (IgG-BFs) produced by T-cell hybrids.
FEBS Lett
; 171(1): 41-5, 1984 Jun 04.
Article
em En
| MEDLINE
| ID: mdl-6609841
Induced and constitutive murine IgG-binding factors (IgG-BFs) have been purified by affinity chromatography from supernatants of T-cells preincubated with or without murine monoclonal IgG1 and IgG2b, respectively. IgG-BF Mr values have been studied by SDS-polyacrylamide gel electrophoresis (PAGE) after treatment with SDS under conditions which do not noticeably alter their immunosuppressive activities on the secondary in vitro IgG antibody response. Suppression was recovered at Mr values of 80000, 40000 and 20000. When induced IgG-BF was tested, the isotype-specific suppressive activity was found only at 40 kDa. The 20-kDa moiety appeared to derive from the 40-kDa component and the material found at 80 kDa exerted non-specific immunosuppressive effects. We conclude therefore that isotype-specific IgG-BF has an apparent Mr of 40000.
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Base de dados:
MEDLINE
Assunto principal:
Linfócitos T
/
Terapia de Imunossupressão
/
Linfocinas
/
Proteínas Secretadas pela Próstata
Limite:
Animals
Idioma:
En
Ano de publicação:
1984
Tipo de documento:
Article