Inhibitory activity on murine granulocytic colony formation of bone marrow cell-conditioned medium obtained from colony-stimulating factor-producing tumor-bearing nude mice.
Cancer Res
; 44(8): 3313-6, 1984 Aug.
Article
em En
| MEDLINE
| ID: mdl-6611199
ABSTRACT
The effects of bone marrow-conditioned medium obtained from colony-stimulating factor-producing tumor-bearing nude mice (G-BM-CM) on mouse and human granulocyte-macrophage colony formation and mouse erythroid colony and burst formation were studied. Addition of G-BM-CM into the mouse granulocyte-macrophage colony-forming system containing colony-stimulating activity more strongly inhibited granulocyte colony formation than did mixed granulocyte-macrophage and macrophage colony formation, while it did not change the number of granulocyte colonies formed by human bone marrow cells stimulated by human granulocyte colony-stimulating activity. Addition of G-BM-CM slightly increased mouse erythroid colony and burst numbers when it was added into an erythroid colony-forming system stimulated by erythropoietin (1 unit/ml), and into the erythroid burst-forming system stimulated by erythropoietin (1 unit/ml) and 7% spleen cell-conditioned medium. These results might indicate that G-BM-CM mainly blocked commitment of mouse granulocyte-macrophage colony-forming cells to granulocytic progeny.
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Base de dados:
MEDLINE
Assunto principal:
Medula Óssea
/
Células-Tronco Hematopoéticas
/
Fatores Estimuladores de Colônias
/
Granulócitos
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
1984
Tipo de documento:
Article