Accelerated onset of viral transcription in adenovirus-infected HeLa cells treated with the tumor promoter 12-O-tetradecanoyl-phorbol-13-acetate.
Mol Cell Biol
; 4(3): 563-6, 1984 Mar.
Article
em En
| MEDLINE
| ID: mdl-6717433
ABSTRACT
When adenovirus type 5-infected HeLa cells were exposed to the tumor promoter 12-O-tetradecanoyl-phorbol-13-acetate, short pulse-labeling with [3H]uridine in vivo and [3H]UTP incorporation by isolated nuclei in vitro were both consistent with a decreased latent period before initiation by RNA polymerase at early viral promoters. Acceleration was not dependent upon concurrent protein synthesis and could not be attributed to rapid entry of virus into the cell nucleus. 12-O-tetradecanoyl-phorbol-13-acetate suppressed the transcription-delay phenotype of the E1a mutant, hr1, without restoring its ability to replicate.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Transcrição Gênica
/
Forbóis
/
Acetato de Tetradecanoilforbol
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Transformação Celular Viral
/
Adenovírus Humanos
Limite:
Humans
Idioma:
En
Ano de publicação:
1984
Tipo de documento:
Article