Molecular cloning of pheR in Escherichia coli K-12.
J Bacteriol
; 152(1): 1-6, 1982 Oct.
Article
em En
| MEDLINE
| ID: mdl-6749801
ABSTRACT
The regulator gene pheR, which in Escherichia coli controls the expression of pheA, the structural gene for chorismate mutase P-prephenate dehydratase, was cloned on to multicopy plasmids directly from the E. coli chromosome; this was achieved with the aid of the tetracycline resistance transposon, Tn10, that had been inserted very close to the pheR gene. Subsequently, pheR was subcloned on a 1.1-kilobase-pair fragment on the plasmid vector pBR322; its position on the plasmid was localized by the method of gamma delta-mediated transpositional inactivation. The pheR gene product was identified in maxicells and found to be a protein of subunit molecular weight 19,000, suggesting that the coding segment of the gene is about 500 nucleotide pairs long.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Oxirredutases
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Prefenato Desidrogenase
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Genes Reguladores
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Clonagem Molecular
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Escherichia coli
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Genes Bacterianos
Idioma:
En
Ano de publicação:
1982
Tipo de documento:
Article