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Changes in the cellular glycosaminoglycans of cultured mastocytoma cells induced by sodium butyrate.
Biochim Biophys Acta ; 627(1): 60-70, 1980 Jan 03.
Article em En | MEDLINE | ID: mdl-6766327
The effect of sodium butyrate on the cellular glycosaminoglycans of cultured mastocytoma p-815-4 cells was investigated using enzymic digestion, electrophoresis, nitrous acid degradation, and sequential partition fractionation. The average cellular glycosaminoglycan content of mastocytoma p-815-4 cells grown in the presence of 2 mM sodium butyrate was ten times as much as that of the control p-815-4 cells. Approximately 90% of the glycosaminoglycans isolated from the control cells and 70% from the butyrate-treated cells were found to be chondroitin 4-sulfate by enzymic digestion. The remainders were chondroitinase ABC-resistant. Hyaluronic acid and dermatan sulfate were not detected in either control cells or butyrate-treated cells. The chondroitinase ABC-resistant fraction of glycosaminoglycans from butyrate-treated cells showed a molar ratio of sulfate to uronic acid of more than 2.0, and provided some physicochemical properties characteristic to reference bovine lung heparin.
Assuntos
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Base de dados: MEDLINE Assunto principal: Butiratos / Sarcoma de Mastócitos / Glicosaminoglicanos Limite: Animals Idioma: En Ano de publicação: 1980 Tipo de documento: Article
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Base de dados: MEDLINE Assunto principal: Butiratos / Sarcoma de Mastócitos / Glicosaminoglicanos Limite: Animals Idioma: En Ano de publicação: 1980 Tipo de documento: Article