Cloning of a nitrogen fixation (nif) gene cluster of Azospirillum brasilense.
Biochimie
; 64(7): 495-502, 1982 Jul.
Article
em En
| MEDLINE
| ID: mdl-6812651
ABSTRACT
Homology was detected between the structural genes for the nitrogenase complex of K. pneumoniae (nifHDK genes) and the total DNA of several Azospirillum strains. Bacteriophage lambda gt 7-ara6 was used to construct a gene bank of A. brasilense strain 7000 DNA and a recombinant phage carrying a 6.7 kb Eco RI fragment, termed AbRI, was selected by hybridization with the K. pneumoniae nif probe. Using heteroduplex analysis the extent of the homology of the AbRI fragment and the K. pneumoniae nif genes was found to be approximately 5 kb. Proteins encoded by the AbRI fragment were examined after infection of E. coli minicells.
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Base de dados:
MEDLINE
Assunto principal:
Clonagem Molecular
/
Cianobactérias
/
Klebsiella pneumoniae
/
Fixação de Nitrogênio
País como assunto:
America do sul
/
Brasil
Idioma:
En
Ano de publicação:
1982
Tipo de documento:
Article