[Determination of lysine residue number, positive charge and molecular lengths of histone H1 and H5 by a method of incomplete succinylation]. / Opredelenie chisla ostatkov lizina, polozhitel'nogo zariada i molekuliarnoi dliny gistonov H1 in H5 metodom nepolnogo suktsinilirovaniia.

ABSTRACT

A simple method for determination of lysine residue number and positive charge of histones H1 and H5 in the acetic acid -- urea system has been developed. The method is based on incomplete succinylation of lysine residues and allows an accurate determination of protein molecular length. The accuracy of the method has been demonstrated by determination of goose and chicken histones H5 and their fragments. The calibrating curve for determination of molecular lengths of histones H1 and H5 has been plotted. Using the incomplete succinylation method, a detailed analysis of Lymantria dispar histone H1 structure has been carried out. The method under discussion can be used for determining lysine residue number, positive charge and molecular length of practically any protein.