Separation of native types I and III collagens and denatured chains by reverse-phase high-performance liquid chromatography.
Biosci Rep
; 3(1): 93-100, 1983 Jan.
Article
em En
| MEDLINE
| ID: mdl-6839013
A simple and effective high-performance-liquid-chromatography system has been developed for the separation of the native forms of Types I and III collagens. This separation is achieved on a commercially available 330-A-pore-size C18 reverse-phase support using a solvent system consisting of ammonium bicarbonate (0.05 M) and trifluoroacetic acid (0.4%) with tetrahydrofuran as the eluting solvent. This simple system can also be applied to the separation of mixtures of the denatured chains of both collagen types.
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Base de dados:
MEDLINE
Assunto principal:
Cromatografia Líquida de Alta Pressão
/
Colágeno
Limite:
Animals
Idioma:
En
Ano de publicação:
1983
Tipo de documento:
Article