Further production and characterization of antibodies reactive with pyrazolone derivatives.
J Immunol Methods
; 35(1-2): 147-55, 1980.
Article
em En
| MEDLINE
| ID: mdl-7204986
ABSTRACT
A sensitive radioimmunoassay for pyrazolone derivatives has been developed. Anti-antipyrine antisera were produced in rabbits by repeated immunization with 4-succinamidoantipyrine coupled to bovine serum albumin. Less than 1 ng of antipyrine could be detected by this procedure. Various substituents on the carbon-4 position of the pyrazolone ring decreased the affinity for the antibody. The concentrations in ng of various pyrazolone derivatives required to inhibit [3H]antipyrine binding by 50% were antipyrine, 6.8; aminopropylon, 8.5; sulpyrine, 35.5; isopropylantipyrine, 1320; and aminopyrine, 2820. The antibody showed no cross-reactivity with any other antipyretics such as pyrazolidine or aniline derivatives. The determination of antipyrine and sulpyrine concentrations in rat serum after i.p administration was also carried out.
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Base de dados:
MEDLINE
Assunto principal:
Pirazóis
/
Anticorpos
Limite:
Animals
Idioma:
En
Ano de publicação:
1980
Tipo de documento:
Article