Determination of plasma catecholamines by means of radioenzymatic labelling and high pressure liquid chromatographic separation.
Naunyn Schmiedebergs Arch Pharmacol
; 315(3): 233-9, 1981 Jan.
Article
em En
| MEDLINE
| ID: mdl-7219572
0.05 ml plasma samples are incubated with 3H-S-adenosylmethionine and catechol-O-methyl-transferase. The resulting methodoxy derivatives are extracted, the extracts separated by high pressure liquid chromatography and the metanephrine fractions collected. Evaluation is performed by liquid scintillation counting of radioactivity in the respective fractions. The following performance criteria are presented: precision, accuracy, detectability (40 pg/ml for adrenaline and noradrenaline, 130 pg/ml for dopamine), linearity and day-by-day variation. Comparison with a standard method shows an excellent correlation for adrenaline and noradrenaline.
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Base de dados:
MEDLINE
Assunto principal:
Catecolaminas
Limite:
Adult
/
Humans
/
Male
Idioma:
En
Ano de publicação:
1981
Tipo de documento:
Article