Enhanced secretion of endothelin by endothelial cells in response to hemoglobin.
Neurol Med Chir (Tokyo)
; 33(11): 739-43, 1993 Nov.
Article
em En
| MEDLINE
| ID: mdl-7506808
ABSTRACT
Confluent cultures of bovine aortic endothelium in serum-free medium were exposed to increasing concentrations (10(-6)-10(-4) M) of freshly prepared erythrocyte lysates (primarily hemoglobin). Hemoglobin increased endothelin-1 secretion into the medium in a dose-dependent manner after 24 hours. The enhanced secretion of endothelin-1 in response to hemoglobin was sustained for 72 hours, suggesting active production and secretion of endothelin-1 rather than release from intracellular pools. Secreted endothelin-1 in the medium was characterized by high-performance liquid chromatography coupled with radioimmunoassay. Endothelin-1, a potent and long-lasting vasoconstrictor, may be one of the causative factors of cerebral vasospasm after subarachnoid hemorrhage. Oxyhemoglobin, derived from periarterial clot, may play an important role in the secretion of endothelin-1 in cerebral vasospasm.
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Base de dados:
MEDLINE
Assunto principal:
Hemoglobinas
/
Endotélio Vascular
/
Endotelinas
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
1993
Tipo de documento:
Article