Radioimmunoassay versus flow cytometric assay to quantify LPS-binding protein (LBP) concentrations in human plasma.
J Immunol Methods
; 171(2): 169-76, 1994 May 16.
Article
em En
| MEDLINE
| ID: mdl-7515085
ABSTRACT
LPS-binding protein (LBP) is an acute phase protein present in plasma, that has been shown to play a major role in sensitizing monocytes to LPS. We describe here two assays to quantify LBP in plasma. The first assay made use of monophosphoryl lipid A to capture LBP in human plasma, and LBP was detected by radiolabeled anti-LBP IgG. The second assay measured LBP by flow cytometry, using LBPs ability to present LPS to the CD14 receptor present on monocytes. Both assays had a similar level of sensitivity, that allowed the quantification of LBP in human plasma.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas de Fase Aguda
/
Glicoproteínas de Membrana
/
Proteínas de Transporte
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
1994
Tipo de documento:
Article