A single-injection protein kinase A-directed antisense treatment to inhibit tumour growth.
Nat Med
; 1(6): 528-33, 1995 Jun.
Article
em En
| MEDLINE
| ID: mdl-7585118
ABSTRACT
Expression of the RI alpha subunit of cAMP-dependent protein kinase type I is enhanced in human cancer cell lines, in primary tumours, in cells after transformation and in cells upon stimulation of growth. We have investigated the effect of sequence-specific inhibition of RI alpha gene expression on in vivo tumour growth. We report that single injection RI alpha antisense treatment results in a reduction in RI alpha expression and inhibition of tumour growth. Tumour cells behaved like untransformed cells by making less protein kinase type I. The RI alpha antisense, which produces a biochemical imprint for growth control, requires infrequent dosing to halt neoplastic growth in vivo.
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Base de dados:
MEDLINE
Assunto principal:
Carcinoma
/
Terapia Genética
/
Oligonucleotídeos Antissenso
/
Neoplasias do Colo
/
Proteínas Quinases Dependentes de AMP Cíclico
/
Isoenzimas
/
Proteínas de Neoplasias
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
1995
Tipo de documento:
Article