Characterization of the nuclear gene encoding mitochondrial aconitase in the marine red alga Gracilaria verrucosa.
Plant Mol Biol
; 28(4): 635-46, 1995 Jul.
Article
em En
| MEDLINE
| ID: mdl-7647296
ABSTRACT
We have cloned a nuclear gene from the marine red alga Gracilaria verrucosa that encodes the complete 779 amino-acid mitochondrial aconitase (m-ACN), the first characterized from a photosynthetic organism. The N-terminal 28 deduced amino acids are predicted to constitute the mitochondrial transit peptide, the first described from a red alga. Putative transcriptional cis-acting elements were identified in the upstream untranslated region. The G. verrucosa m-ACN gene (m-ACN) is present in a single copy and is located ca. 1.5 kb upstream from the single-copy polyubiquitin gene. The single spliceosomal intron is located near the 5' end of the region encoding the mature m-ACN in precisely the same location and phase as intron 2 in Caenorhabditis elegans m-ACN; sequences at its 3' and 5' splice junctions and at the predicted lariat branch point conform well to the eukaryote consensus sequences. Multiple protein-sequence alignment of m-ACN, bacterial aconitase (b-ACN) and iron-responsive element-binding protein (IRE-BP), and phylogenetic analyses, revealed that m-ACN does not share a recent common ancestry with either b-ACN or IRE-BP.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Aconitato Hidratase
/
Compartimento Celular
/
Núcleo Celular
/
Rodófitas
/
Mitocôndrias
Idioma:
En
Ano de publicação:
1995
Tipo de documento:
Article