Polyclonal antibodies against malondialdehyde-modified proteins: characterization and application in study of in vitro lipid peroxidation of cellular membranes.
Biochem Mol Biol Int
; 36(2): 355-64, 1995 Jun.
Article
em En
| MEDLINE
| ID: mdl-7663439
ABSTRACT
In order to develop an immunofluorescent technique able to detect the cellular damage induced by lipid peroxidation, we produced rabbit polyclonal antibodies (AbAIP) against malondialdehyde (MDA)-modified lysozyme. These antibodies specifically recognized all the MDA-treated proteins tested, but not native proteins or proteins treated by other aldehydes. Moreover, we showed by an enzyme-linked immunosorbent assay (ELISA) that the amount of immunoreactive protein in MDA-treated erythrocytes was proportional to the concentration of MDA added, suggesting that this ELISA measurement may represent a quantitative determination of peroxidative protein alterations. In addition, when coupled to an indirect fluorochrome antibody conjugate [fluorescein isothiocyanate (FITC)], these antibodies permitted precise localization of the modified proteins within the membranes of peroxidized cells.
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Base de dados:
MEDLINE
Assunto principal:
Peroxidação de Lipídeos
/
Muramidase
/
Membrana Eritrocítica
/
Malondialdeído
/
Proteínas de Membrana
/
Anticorpos
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
1995
Tipo de documento:
Article