A cytosolic protein tyrosine kinase activity is induced by follicle stimulating hormone and insulin like growth factor-I in human granulosa cells.
Endocrinology
; 136(10): 4705-8, 1995 Oct.
Article
em En
| MEDLINE
| ID: mdl-7664693
ABSTRACT
Follicle stimulating hormone (FSH) induces estradiol (E2) production in rat, porcine and human granulosa cells with a concomitant increase in cAMP. In human granulosa cells insulin like growth factor-I (IGF-I) induces E2 production without cAMP accumulation. In the current study we report that IGF-I and FSH effects on aromatase activity both involve activation of a cytosolic soluble protein tyrosine kinase (CytPTK). This FSH and IGF-I stimulated CytPTK activity was blocked by AG-82 (a tyrosine kinase inhibitor) and by staurosporine (STS) (a non specific protein kinase inhibitor) at concentrations which inhibited E2 production. These new findings strengthen the concept of fail-safe mechanism in E2 production in human granulosa cells by an involvement of tyrosine kinase(s) activity downstream of cAMP formation and protein kinase A (PKA) activation.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas Tirosina Quinases
/
Fator de Crescimento Insulin-Like I
/
Hormônio Foliculoestimulante
/
Células da Granulosa
Limite:
Female
/
Humans
Idioma:
En
Ano de publicação:
1995
Tipo de documento:
Article