A BDNF autocrine loop in adult sensory neurons prevents cell death.
Nature
; 374(6521): 450-3, 1995 Mar 30.
Article
em En
| MEDLINE
| ID: mdl-7700353
ABSTRACT
During the initial phase of their development, sensory neurons of the dorsal root ganglion (DRG) require target-derived trophic support for their survival, but as they mature they lose this requirement. Because many of these neurons express BDNF (brain-derived neurotrophic factor) messenger RNA, we hypothesized that BDNF might act as an autocrine survival factor in adult DRG neurons, thus explaining their lack of dependence on exogenous growth factors. When cultured adult DRG cells were treated with antisense oligonucleotides to BDNF, expression of BDNF protein was reduced by 80%, and neuronal survival was reduced by 35%. These neurons could be rescued by exogenous BDNF or neurotrophin-3, but not by other growth factors. Similar results were obtained with single-neuron microcultures, whereas microcultures derived from mutant mice lacking BDNF were unaffected by antisense oligonucleotides. Our results strongly support an autocrine role for BDNF in mediating the survival of a subpopulation of adult DRG neurons.
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Base de dados:
MEDLINE
Assunto principal:
Morte Celular
/
Proteínas do Tecido Nervoso
/
Neurônios Aferentes
Limite:
Animals
Idioma:
En
Ano de publicação:
1995
Tipo de documento:
Article