Production of the chimeric-binding protein, maltose-binding protein-protein A, by gene fusion.
J Biotechnol
; 38(3): 263-8, 1995 Jan 31.
Article
em En
| MEDLINE
| ID: mdl-7765875
ABSTRACT
A fusion protein between maltose-binding protein (MBP) and staphylococcal protein A (SpA) was genetically produced. The gene fusion plasmid, pMALPA2, was constructed by inserting the protein A gene into an expression vector of maltose-binding protein in frame, and was expressed efficiently in Escherichia coli. The resulting fusion protein of molecular mass 65 kDa, retained the activity of both MBP and SpA (binding capability to amylose and immunoglobulin G). This chimeric-binding protein was used as an adhesive molecule for immobilization of antibodies to a solid-phase surface for enzyme immunoassay. An enzyme immunoassay was performed with the fusion protein, and human IgG was determined in the concentration range from 10(-4) to 10(-6) g ml-1.
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Base de dados:
MEDLINE
Assunto principal:
Proteína Estafilocócica A
/
Proteínas de Transporte de Monossacarídeos
/
Proteínas de Transporte
/
Clonagem Molecular
/
Transportadores de Cassetes de Ligação de ATP
/
Proteínas de Escherichia coli
Limite:
Humans
Idioma:
En
Ano de publicação:
1995
Tipo de documento:
Article