Mechanism of interaction between cisplatin and human recombinant interferon gamma in human ovarian-cancer cell lines.
Int J Cancer
; 61(5): 643-8, 1995 May 29.
Article
em En
| MEDLINE
| ID: mdl-7768637
ABSTRACT
Human ovarian carcinoma cells (2008 and its cisplatin-resistant sub-line 2008/C13*) were sensitized to cisplatin by treatment with human recombinant gamma interferon (IFN gamma). IFN gamma produced no significant change in the uptake of CDDP. Exposure of 2008 and 2008/C13* cells to IFN gamma resulted in a time-dependent decrease of cellular glutathione and total glutathione-S-transferase activity, principally the pi isoform. By contrast, the treatment of 2008 and 2008/C13* cell lines with IFN gamma induced rather than suppressed metallothionein IIA mRNA levels. IFN gamma changed neither the formation of total platinum-DNA adducts, nor DNA repair. A significant decrease in c-erbB-2 expression was observed both in sensitive and in resistant cell lines after treatment with IFN gamma, and this decrease was dose-dependent. Our results indicate that the mechanism of IFN gamma-induced sensitization in human ovarian-cancer cell lines is multifactorial.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Neoplasias Ovarianas
/
Interferon gama
/
Cisplatino
Limite:
Female
/
Humans
Idioma:
En
Ano de publicação:
1995
Tipo de documento:
Article