Determination of MK-383, a non-peptide fibrinogen receptor antagonist, in human plasma and urine by radioimmunoassay.
J Pharm Biomed Anal
; 12(8): 1047-53, 1994 Aug.
Article
em En
| MEDLINE
| ID: mdl-7819379
ABSTRACT
MK-383 is a novel, non-peptide fibrinogen receptor antagonist. A sensitive and specific radioimmunoassay has been developed for the determination of this drug candidate in plasma and urine. The immunogen was prepared by coupling to albumin via the N-hydroxysuccinimide ester from which the radioligand was also prepared by reaction with [I125]iodotyrosine. The method was specific and no immunoreactive material other than the parent drug was detectable in plasma and urine from dosed volunteers. This direct assay, using 5 microliters of plasma or 0.5 microliter of urine, is sensitive to 1 and 10 ng ml-1, respectively, without matrix interference and has sufficient sensitivity, specificity, accuracy, and precision for the analysis of clinical samples.
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Base de dados:
MEDLINE
Assunto principal:
Tirosina
/
Glicoproteínas da Membrana de Plaquetas
/
Fibrinolíticos
Limite:
Animals
/
Female
/
Humans
Idioma:
En
Ano de publicação:
1994
Tipo de documento:
Article