Effect of N,N-diethyldithiocarbamate on ionomycin-induced increase in oxidation of cellular 2',7'-dichlorofluorescin in dissociated cerebellar neurons.
Brain Res
; 660(1): 158-61, 1994 Oct 10.
Article
em En
| MEDLINE
| ID: mdl-7827993
ABSTRACT
Effect of N,N-diethyldithiocarbamate (DDC), an inhibitor for cytosolic superoxide dismutase, on an ionomycin-induced increase in oxidative metabolism was examined in cerebellar neurons dissociated from the rats, using a flow cytometer and 2',7'-dichlorofluorescin diacetate and fluo-3-AM, fluorescent dyes for intracellular hydrogen peroxide and Ca2+, respectively. DDC reduced the ionomycin-induced augmentation of 2',7'-dichlorofluorescin fluorescence in a dose-dependent manner. DDC did not affect cellular content of 2',7'-dichlorofluorescin and ionomycin-induced increase in intracellular Ca2+ concentration. Results indicate that ionomycin increases the formation of superoxide anion in brain neuron.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Ionomicina
/
Cerebelo
/
Ditiocarb
/
Fluoresceínas
/
Neurônios
Limite:
Animals
Idioma:
En
Ano de publicação:
1994
Tipo de documento:
Article