Evidence for an intermediate in the enzymatic formation of uroporphyrinogen III.

Pichon, C; Atshaves, B P; Stolowich, N J; Scott, A I

Pichon, C; Atshaves, B P; Stolowich, N J; Scott, A I.

Afiliação

Pichon C; Department of Chemistry, Texas A&M University, College Station 77843-3255.

Bioorg Med Chem ; 2(3): 153-68, 1994 Mar.

Article em En | MEDLINE | ID: mdl-7922129

ABSTRACT

ABSTRACT

Evidence for an azafulvene intermediate in the enzymatic formation of Uroporphyrinogen III has been obtained. Using conditions to slow down the enzyme activity (high pH, low temperature), the transient species was trapped with ammonium ions as aminomethylbilane and with sodium borohydride as methylbilane, and observed by 13C-NMR.

Assuntos

Uroporfirinogênios/biossíntese; Escherichia coli/enzimologia; Escherichia coli/genética; Genes Bacterianos; Concentração de Íons de Hidrogênio; Técnicas In Vitro; Espectroscopia de Ressonância Magnética; Estrutura Molecular; Proteínas Recombinantes/biossíntese; Proteínas Recombinantes/química; Proteínas Recombinantes/genética; Temperatura; Uroporfirinogênio III Sintetase/metabolismo; Uroporfirinogênios/química; Uroporfirinogênios/genética; Uroporfirinogênios/metabolismo

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