A miniaturized counting technique for anaerobic bacteria.
Can J Microbiol
; 22(12): 1728-33, 1976 Dec.
Article
em En
| MEDLINE
| ID: mdl-795533
ABSTRACT
A miniaturized counting technique gave results as good as the pour-plate and Most Probable Number (MPN) techniques for enumeration of clostridia spp. and anaerobic isolates from the gut. Highest counts were obtained when ascorbic acid (1%) and dithiothreitol (0.015%) were added to the reinforced clostridial medium used for counting. This minimized the effect of exposure to air before incubation. The miniature technique allowed up to 40 samples to be plated and incubated in one McIntosh-Filde's-type anaerobic jar, compared with 3 or 4 by the normal pour plate.
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Base de dados:
MEDLINE
Assunto principal:
Bactérias
/
Técnicas Bacteriológicas
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
Idioma:
En
Ano de publicação:
1976
Tipo de documento:
Article