Differences in Ca2+ permeability of AMPA-type glutamate receptor channels in neocortical neurons caused by differential GluR-B subunit expression.
Neuron
; 12(6): 1281-9, 1994 Jun.
Article
em En
| MEDLINE
| ID: mdl-8011338
ABSTRACT
Fast excitatory synaptic transmission in the CNS is mediated by AMPA-type glutamate receptor (GluR) channels. Heterologous expression suggested that the Ca2+ permeability of these receptors critically depends on the subunit composition. Using patch-clamp techniques in brain slices, we found that the Ca2+ permeability of native AMPA-type GluRs was markedly higher in nonpyramidal (PCa/PK approximately 0.63) than in pyramidal (PCa/PK approximately 0.05) neurons of rat neocortex. Analysis of mRNA in single cells indicated that the relative abundance of GluR-B-specific mRNA was significantly lower in nonpyramidal (GluR-B/GluR-non-B approximately 0.3) than in pyramidal (GluR-B/GluR-non-B approximately 3) cells. This suggests that differences in relative abundance of GluR-B-specific mRNA generate functional diversity of AMPA-type GluRs in neurons with respect to Ca2+ permeability.
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Base de dados:
MEDLINE
Assunto principal:
Córtex Visual
/
Canais de Cálcio
/
Expressão Gênica
/
Córtex Cerebral
/
Cálcio
/
Receptores de Glutamato
/
Receptores de AMPA
/
Células Piramidais
/
Neurônios
Limite:
Animals
Idioma:
En
Ano de publicação:
1994
Tipo de documento:
Article