Identification of the DNA-interacting sites of proteins: microsequencing of the peptides cross-linked to 5-bromouracil substituted DNA.
Biochimie
; 76(2): 129-32, 1994.
Article
em En
| MEDLINE
| ID: mdl-8043648
ABSTRACT
Photochemical induced cross-links between protein and nucleic acids are useful tools in the study of the protein-DNA interactions. The substitution of thymine by 5-bromouracil in DNA increases the photocross-linking yield, and reduces the direct damages to both DNA and proteins. Using the lac repressor-DNA non-specific interaction system, we have developed a procedure to identify the interaction site on the protein. Sensitive, accurate and inexpensive in time and material, this procedure is based on the possibility of sequencing peptides in the presence of a large excess of DNA. The obtained result (the implication of His 29) agrees with previous work.
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Base de dados:
MEDLINE
Assunto principal:
Bromouracila
/
DNA
/
Sequência de Aminoácidos
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Idioma:
En
Ano de publicação:
1994
Tipo de documento:
Article