Improved shuttle vectors for Haloferax volcanii including a dual-resistance plasmid.
Gene
; 146(1): 117-21, 1994 Aug 19.
Article
em En
| MEDLINE
| ID: mdl-8063095
ABSTRACT
Two new Haloferax-Escherichia shuttle vectors are described, pMDS20 and pMLH3. These vectors contain the E. coli ColE1 plasmid ori region and ampicillin-resistance(ApR)-conferring bla gene, and the Haloferax pHK2 replicon region and novobiocin-resistance(NbR)-encoding gyrB gene, enabling maintenance and selection in both hosts. Plasmid pMLH3 has, in addition, a H. volcanii mevinolin-resistance (MvR) determinant and restriction sites allowing insertional inactivation of either marker, to facilitate the identification of Haloferax transformants harbouring cloned sequences. Sequencing of gyrA, within the NbR determinant, and the pHK2 ori region has been completed so the complete sequence of both pMDS20 and pMLH3 is now known.
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Base de dados:
MEDLINE
Assunto principal:
Plasmídeos
/
DNA Topoisomerases Tipo II
/
Genes Bacterianos
/
Vetores Genéticos
/
Halobacterium
Idioma:
En
Ano de publicação:
1994
Tipo de documento:
Article