Interleukin-4 protects double-negative and CD4 single-positive thymocytes from dexamethasone-induced apoptosis.
Blood
; 81(5): 1352-8, 1993 Mar 01.
Article
em En
| MEDLINE
| ID: mdl-8095166
ABSTRACT
Glucocorticoid hormones (GCH) and anti-CD3 monoclonal antibodies (MoAbs) induce in mouse thymocytes and T-cell tumor lines an active process of cell death called apoptosis. Interleukins (IL), including IL-1 and IL-2, have been reported to inhibit such apoptosis. In this study we show that IL-4 also reduced the DNA fragmentation characteristic of dexamethasone (DEX)-induced apoptosis in thymocytes. This effect, studied in both time-course and dose-response experiments, was also detected at low IL-4 concentrations (1 U/mL) and against high DEX levels (10(-7) mol/L). The effect of IL-4 was blocked by an anti-IL-4 but not by an anti-IL-1 alpha MoAb, and was thus both specific and direct. Phenotypic analysis showed that IL-4 protects predominantly CD4-CD8- and CD4+CD8- cells. Our findings suggest that intrathymic T-cell development may be influenced by IL-4.
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Base de dados:
MEDLINE
Assunto principal:
Dexametasona
/
Linfócitos T CD4-Positivos
/
Subpopulações de Linfócitos T
/
Interleucina-4
/
Apoptose
Limite:
Animals
Idioma:
En
Ano de publicação:
1993
Tipo de documento:
Article