Interleukin 7-induced expression of specific T cell receptor gamma variable region genes in murine fetal liver cultures.
J Exp Med
; 178(6): 2201-6, 1993 Dec 01.
Article
em En
| MEDLINE
| ID: mdl-8245791
ABSTRACT
We previously reported that culture of murine fetal liver (FL) cells with interleukin 7 (IL-7) results in expression of high levels of T cell receptor (TCR) gamma transcripts by a population of cells expressing Thy-1 and Pgp-1, suggesting that IL-7 promotes the growth and/or differentiation of pre-T cells. We demonstrate herein that culture of FL cells for 7 d with IL-7 caused the rearrangement and expression of TCR gamma variable (V) region genes V gamma 4 and V gamma 6, but not V gamma 5 or V gamma 7. Since this effect was not blocked by hydroxyurea, it appeared to represent induction of expression of these genes by IL-7 rather than expansion of a preexisting positive population. We also show that IL-7 induced RAG-1 and RAG-2 mRNA expression by FL cells. These data provide evidence that specific TCR gamma/delta V region genes can be rearranged and expressed by T lineage cells before their migration to the thymus, in response to IL-7.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Linfócitos T
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Interleucina-7
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Receptores de Antígenos de Linfócitos T gama-delta
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Rearranjo Gênico da Cadeia gama dos Receptores de Antígenos dos Linfócitos T
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Proteínas de Homeodomínio
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Proteínas de Ligação a DNA
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Fígado
Limite:
Animals
Idioma:
En
Ano de publicação:
1993
Tipo de documento:
Article