Characterization and chromosomal localization of the human proto-oncogene BMI-1.
Hum Mol Genet
; 2(10): 1597-603, 1993 Oct.
Article
em En
| MEDLINE
| ID: mdl-8268912
ABSTRACT
The proto-oncogene bmi-1 is frequently activated by Moloney murine leukemia proviral insertions in E mu-myc transgenic mice1,2. Using a mouse bmi-1 cDNA probe a transcript of 3.3 kb was detected on Northern blots of human Burkitt's lymphoma cell lines. We have isolated and sequenced cDNA clones from a human erythroleukemia cell line (K562) derived cDNA library, using different mouse bmi-1 cDNA fragments as a probe. Analysis of genomic BMI-1 sequences reveals a gene structure which is very similar to that of the mouse, consisting of at least 10 exons. The human cDNA is 3203 bp in length and shows 86% identity to the mouse nucleotide sequence. The open reading frame encodes a protein of 326 amino acids which shares 98% identity to the amino acid sequence of mouse bmi-1 protein. In vitro translation experiments show that human cDNA derived RNA translates into a protein with a mobility of 44-46 kD on SDS polyacrylamide gels. Fluorescence in situ hybridization (FISH) on metaphase chromosome spreads located the human BMI-1 gene to the short arm of chromosome 10 (10p13), a region known to be involved in translocations in various leukemias.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas Repressoras
/
Cromossomos Humanos Par 10
/
Proto-Oncogenes
/
Proteínas Nucleares
/
Proteínas Proto-Oncogênicas
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Proteínas de Drosophila
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Proteínas de Ligação a DNA
/
Genes
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
1993
Tipo de documento:
Article