Dithiothreitol stimulates the activity of the plasma membrane aminophospholipid translocator.
Biochim Biophys Acta
; 1150(1): 57-62, 1993 Jul 25.
Article
em En
| MEDLINE
| ID: mdl-8334138
ABSTRACT
Metabolic depletion induces human erythrocytes to crenate, a shape change that is reversed when ATP is regenerated by nutrient supplementation. In the presence of the sulfhydryl reducing agent dithiothreitol (DTT), this shape reversal is exaggerated, proceeding beyond normal discoid morphology to stomatocytic forms. DTT-induced stomatocytosis does not correlate consistently with alterations in cell ATP, spectrin phosphorylation, or phosphoinositide metabolism (Truong, H.-T.N., Ferrell, J.E., Jr. and Huestis, W.H. (1986) Blood 67, 214-221). The effect of DTT on outer-to-inner-monolayer transport of aminophospholipids was examined by monitoring shape changes induced by dilauroylphosphatidylserine (DLPS). Stomatocytosis induced by transport of this exogenous lipid to the membrane inner monolayer is accelerated and exaggerated by DTT. The effect of DTT on DLPS translocation is reversible and temperature dependent, consistent with the intervention of reducing agents in the activity of the aminophospholipid translocator. These findings bear on the relationship between cell redox status and shape regulation.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas de Transporte
/
Proteínas de Transferência de Fosfolipídeos
/
Tamanho Celular
/
Ditiotreitol
/
Membrana Eritrocítica
/
Proteínas de Membrana
Limite:
Humans
Idioma:
En
Ano de publicação:
1993
Tipo de documento:
Article