A cosmid contig and high resolution restriction map of the 2 megabase region containing the Huntington's disease gene.
Nat Genet
; 4(2): 181-6, 1993 Jun.
Article
em En
| MEDLINE
| ID: mdl-8348156
The quest for the mutation responsible for Huntington's disease (HD) has required an exceptionally detailed analysis of a large part of 4p16.3 by molecular genetic techniques, making this stretch of 2.2 megabases one of the best characterized regions of the human genome. Here we describe the construction of a cosmid and P1 clone contig spanning the region containing the HD gene, and the establishment of a detailed, high resolution restriction map. This ordered clone library has allowed the identification of several genes from the region, and has played a vital role in the recent identification of the Huntington's disease gene. The restriction map provides the framework for the detailed analysis of a region extremely rich in coding sequences. This study also exemplifies many of the strategies to be used in the analysis of larger regions of the human genome.
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Base de dados:
MEDLINE
Assunto principal:
Cromossomos Humanos Par 4
/
Cosmídeos
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Mapeamento por Restrição
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Biblioteca Gênica
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Doença de Huntington
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Genes
Limite:
Humans
Idioma:
En
Ano de publicação:
1993
Tipo de documento:
Article