Photoaffinity labeling of the NMDA receptor.
Eur J Pharmacol
; 246(2): 179-80, 1993 Jul 15.
Article
em En
| MEDLINE
| ID: mdl-8375463
ABSTRACT
The structure of NMDA receptors in situ has been probed with the novel photoaffinity ligand 125I-CGP 55802A. By covalently linking the radioactive high-affinity photolabel to NMDA receptors in bovine brain we have identified a protein of 175 kDa associated with the binding site for NMDA receptor agonists and competitive antagonists. Based on its molecular size the photolabeled protein is likely to correspond to the NR2A and/or NR2B subunit. The photoaffinity ligand will permit the assessment of regulatory changes in NMDA receptor subunit expression.
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Base de dados:
MEDLINE
Assunto principal:
Receptores de N-Metil-D-Aspartato
Limite:
Animals
Idioma:
En
Ano de publicação:
1993
Tipo de documento:
Article